Comet assay in myelodysplastic syndromes

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Comet assay in myelodysplastic syndromes

The comet assay (single-cell gel electrophoresis) has been established as a simple, rapid, flexible and sensitive method of detecting DNA damage in single cells(1,2). Cells embedded in agarose on a microscope slide are lysed with detergent. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the intensity of the comet tail relative to the hea...

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Myelodysplastic syndromes.

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ژورنال

عنوان ژورنال: Revista Brasileira de Hematologia e Hemoterapia

سال: 2012

ISSN: 1516-8484

DOI: 10.5581/1516-8484.20120080